Comparison and optimization of conjugated linoleic acid production by Lactobacillus plantarum and Lactobacillus plantarum subsp. plantarum

Kouchak Yazdi, Z.; Alemzadeh, I.; Vossoughi, M.

doi:10.24200/sci.2017.4111

Comparison and optimization of conjugated linoleic acid production by Lactobacillus plantarum and Lactobacillus plantarum subsp. plantarum

Document Type : Research Article

Authors

Z. Kouchak Yazdi

I. Alemzadeh

M. Vossoughi

Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran

10.24200/sci.2017.4111

Abstract

Conjugated Linoleic Acid (CLA) was produced from castor oil using washed cells of Lactobacillus plantarum PTCC 1058 and Lactobacillus plantarum subsp. plantarum PTCC1745 as the catalyst. Under the optimal reaction conditions, the washed cells of Lactobacillus plantarum PTCC1058 produced 1661.26 mg of CLA/L reaction mixture (36% yield of production) from 4.6 mg/ml of castor oil after using 15% (w/v) cell for 121 h.
The resulting CLA was mixture of two CLA isomers, cis-9, trans-11 (or trans-9, cis- 11)-octadecadienoic acid (CLA1, 44% of total CLA) and trans-10, cis-12-octadecadienoic acid (CLA2, 46% of total CLA). The total production of CLA is extracellular in all of the reactions performed with Lactobacillus plantarum PTCC1058. Also, Lactobacillus plantarum PTCC1745 produced 1590.31 mg of CLA/L reaction mixture (16.5% production
yield) from 9.6 mg/ml of castor oil after using 15% (w/v) cell for 121 h. Therefore, Lactobacillus plantarum PTCC1058 was suggested for CLA production due to its more production ability and CLA extracellular production.

Keywords

Conjugated linoleic acid

Castor oil

Lactobacillus plantarum

optimization

Washed cell

Subjects