A Computational Model for Estimation of Mechanical Parameters in Chemotactic Endothelial Cells


Mechanical Engineering School, Sharif University of Technology, Tehran, Iran


A cell migration numerical simulation is presented to mimic the motility of endothelial cells subjected to the concentration gradients of Forebrain embryonic cortical neuron conditioned medium (CM). This factor was previously shown to induce the directional chemotaxis of endothelial cells with over-expressed G protein coupled receptor 124 (GPR 124). A cell simulator program incorporates basic elements of the cell cytoskeleton including membrane, nucleus and cytoskeletons. The developed 2D cell model is capable of responding to concentration gradients of biochemical factors by changing the cytoskeleton arrangement. Random walk force, cell drag force and the cell inertial effects are also implemented into the cell migration to complete the simulation of the phenomenon.  The obtained results of cell migration were calibrated with experimental cell chemotaxis data. This model can be implemented for prediction of cell behavior during cell chemotaxis and also it provides a powerful tool to explain the cell migration phenomenon mechanistically.