The present study reports a simple, rapid, easy, eco-friendly and cost efficient method for green synthesis of silver nanoparticles (AgNPs)using pollen extract of rose flower as reducer and stabilizer. AgNPs synthesis was performed at room temperature in solutions by reduction rapidly takes place for 15 min and the synthesized AgNPs were stable for a long time. Moreover highlights for this method are avoiding the utilization of toxic organic solvents and using nontoxic water as the solvent for pollen extract from rose flower. Microscopy and UV–Vis spectroscopy confirmed the formation of nanoparticles as well as the colloid of silver samples. Surface plasmon resonance (SPR) peaks in UV–Vis spectra were observed at 427 nm indicated the formation of poly dispersive AgNPs using pollen of rose flower. The synthesized AgNPs were mostly spherical in shape with an average size of 12 nm. It shows the significant antibacterial efficacy against Staphylococcus aureus (S. aureus),Escherichia coli (E. coli), Bacillus cereus (B. cereus) and Enterococcus faecalis (E. faecalis)by disk diffusion method using Mueller-Hinton Agar. From the results, it is suggested that green synthesized AgNPs could be used effectively in cosmetics, foods and medical applications