A Sensitive Colorimetric Detection of Ascorbic Acid in Pharmaceutical Products Based on Formation of Anisotropic Silver Nanoparticles


1 Department of Chemistry,Sharif University of Technology

2 Department of Chemistry,University of Leeds

3 Department of Chemistry,University of Kerman


A sensitive colorimetric method for the detection of ascorbic acid was proposed in this
research based on the reduction of silver ions by ascorbic acid in the presence of citrate-stabilized silver
seeds, additional trisodium citrate and a polymer such as polyvinylpyrrolidone. The color of the stable sol
is controlled by varying the concentration of trisodium citrate (TSC), polyvinylpyrrolidone, silver nitrate
and silver seeds. The reduction of Ag+ to triangle silver nanoparticles (Ag-NPs) by ascorbic acid in the
presence of trisodium citrate (TSC) and silver seeds produced two very intense surface plasmon resonance
peaks of Ag-NPs. The plasmon absorbance of Ag-NPs allows the quantitative spectrophotometric detection
of the ascorbic acid. The calibration curve derived from the changes in absorbance at  = 427 nm was
linear, with the concentration of ascorbic acid in the range of 6:010????6 to 8:010????5 M. The method was
applied to the determination of ascorbic acid in pharmaceutical formulations with satisfactory results.