Comparison of Solid Substrate and Submerged Fermentation for Chitosan Production by Aspergillus niger


1 Department of Chemical and Petrochemical Engineering,Sharif University of Technology

2 Biochemical and Bioenvironmental Research Center,Sharif University of Technology


Production yield of solid-state (SSF) and submerged fermentation (SMF) on chitosan from
Aspergillus niger was investigated. A. niger BBRC 20004 was grown on soybean residue and Sabouraud
Dextrose Broth medium (2% glucose). Chitosan was extracted from the fungal mycelia using hot alkaline
and acid treatment. Soybean residue at a moisture content of 37%, and 8.4  0.26% of nitrogen content
produced the highest amount of chitosan, 17.053  0.95 g/kg dry substrate after 12 days. Also, chitosan
was extracted from A. niger on Sabouraud Dextrose Broth medium in submerged fermentation (0.8455 g/l
after 12 days of cultivation). The yield of chitosan isolated in SSF was about 15-20 times more than in
SMF (Submerged Fermentation).
Keyword: Chitosan; Soybean residue; Solid-State Fermentation (SSF); Submerged Fermentation (SMF);
Sabouraud Dextrose Broth (SDB); Glucose; Aspergillus niger.