1
Department of Chemical and Petroleum Engineering,Sharif University of Technology
2
Department of Civil Engineering,Sharif University of Technology
3
Department of Chemical Engineering,Sharif University of Technology
Abstract
The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous
medium was investigated. The parameters studied were: time course of amoxicillin production,
concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6-
APA) and the e ect of enzyme (PGA) content and pH, under variable and constant conditions and
temperature variations. In the study of two substrate concentration on amoxicillin production, impressive
results were obtained for a 1/3 ratio of 6-amino penicillanic acid (6-APA) and hydroxyl-phenylglycine
methyl ester (HPGM). The synthesis of amoxicillin was preferable at constant pH rather than a variable
one. Other optimal conditions obtained were: enzyme concentration: 5 g/L with 100U, process time: 480
min and temperature: 35C. The yield for amoxicillin synthesis under prescribed conditions showed up to
50%.
Alemzadeh, I., Borghei, S., & Roostaazad, R. (2010). Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase. Scientia Iranica, 17(1), -.
MLA
I. Alemzadeh; S.M. Borghei; R. Roostaazad. "Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase". Scientia Iranica, 17, 1, 2010, -.
HARVARD
Alemzadeh, I., Borghei, S., Roostaazad, R. (2010). 'Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase', Scientia Iranica, 17(1), pp. -.
VANCOUVER
Alemzadeh, I., Borghei, S., Roostaazad, R. Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase. Scientia Iranica, 2010; 17(1): -.
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