Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase


1 Department of Chemical and Petroleum Engineering,Sharif University of Technology

2 Department of Civil Engineering,Sharif University of Technology

3 Department of Chemical Engineering,Sharif University of Technology


The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous
medium was investigated. The parameters studied were: time course of amoxicillin production,
concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6-
APA) and the e ect of enzyme (PGA) content and pH, under variable and constant conditions and
temperature variations. In the study of two substrate concentration on amoxicillin production, impressive
results were obtained for a 1/3 ratio of 6-amino penicillanic acid (6-APA) and hydroxyl-phenylglycine
methyl ester (HPGM). The synthesis of amoxicillin was preferable at constant pH rather than a variable
one. Other optimal conditions obtained were: enzyme concentration: 5 g/L with 100U, process time: 480
min and temperature: 35C. The yield for amoxicillin synthesis under prescribed conditions showed up to