Biogenic synthesis of silver nanoparticles from Lonicera caprifolium extract: characterization and evaluation of antibacterial properties against Pectobactrrium carotovorum subsp carotovorum (Pcc) (the causal agent of bacterial soft rot in vegetables)

Firozeh, Sahar; Halimi Khalilabad, Majid; Taleghani, Akram; Akbari, Reza; Nadaf, Mohabat

doi:10.24200/sci.2025.64903.9210

Biogenic synthesis of silver nanoparticles from Lonicera caprifolium extract: characterization and evaluation of antibacterial properties against Pectobactrrium carotovorum subsp carotovorum (Pcc) (the causal agent of bacterial soft rot in vegetables)

Articles in Press

Document Type : Research Article

Authors

Sahar Firozeh ¹

Majid Halimi Khalilabad ²

Akram Taleghani ¹

Reza Akbari ¹

Mohabat Nadaf ³

¹ Department of Chemistry,Faculty of Science, Gonbad Kavous University,Gonbad,Iran

² Department of Chemistry, Kosar University of Bojnord, Bojnord, Iran

³ Department of Molecular and Cell Biology, Kosar University of Bojnord, Bojnord, Iran

10.24200/sci.2025.64903.9210

Abstract

Nanoscience enables the manipulation of matter at the nanoscale, with biologically synthesized nanoparticles offering applications in medicine, optics, and agriculture. In this study, the antibacterial (phytopathogenic) effect of silver nano particles (AgNPs) produced using the aqueous extract of the aerial parts (flowers and leaves) of the Lonicera caprifolium was investigated using the Agar well diffusion method. UV-Vis spectroscopy (300–700 nm) confirmed AgNP synthesis, while SEM/TEM revealed spherical nanoparticles sized 10–50 nm (flower extract) and 10–80 nm (leaf extract). FTIR identified organic compounds involved in reduction. Based on the results of this research, the nanoparticles produced by the aqueous extract of the Lonicera caprifolium have effective antimicrobial activity against Pectobactrrium carotovorum subsp carotovorum (Pcc). In this way, the aerial parts (flowers and leaves) of Lonicera caprifolium can be used as a useful biological resource for the synthesis of AgNPs on an industrial scale at a very low cost. This research aims to contribute valuable insights into the potential use of plant-mediated silver nanoparticles as effective antibacterial agents for combating phytopathogens and addressing challenges in agricultural disease management.

Keywords

nanosilver

Lonicera caprifolium

antibacterial

Pectobactrrium carotovorum subsp carotovorum

phytopathogen

Subjects