Antibacterial properties of zinc oxide nanoparticles on Pseudomonas aeruginosa (ATCC 27853)

Document Type : Article


1 Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia

2 Department of Biotechnology, Faculty of Health and Life Sciences, INTI International University, Nilai, Negeri Sembilan, Malaysia

3 Centre for Ocean Research, (DST-FIST sponsored Centre), Sathyabama Institute of Science and Technology, Chennai, India

4 Department of Mechatronics & Biomedical Engineering, Lee Kong Chian Faculty of Engeneering and Sciences, Universiti Tunku Abdul Rahman, Bandar Sungai Long, 43000, Malaysia

5 Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Bandar Sungai Long, 43000, Malaysia


The involvement of nanotechnology has brought advancements in the environmental
and medical applications. Recently, zinc oxide nanoparticle (ZnO NP) is commonly used to
treat a wide range of bacterial and fungal skin infections due to its antimicrobial property.
This investigation was intended to study the antimicrobial effect of ZnO NP on Pseudomonas
aeruginosa by testing the bacterial inhibition and the morphological damages caused by ZnO
NP on P. aeruginosa. The results of the study at 24 h exhibited a typical dose dependant and
significant (p> 0.05) inhibition on the growth of P. aeruginosa treated with 5 to 150 μg/mL
of ZnO NP. The polysaccharides and polypeptides from P. aeruginosa cell wall were found to
be associated to the attachment of ZnO NPs on bacterial cells as illustrated in the Fourier
transform infrared (FTIR) spectrum. Furthermore, the scanning electron microscopy (SEM)
images displayed the surface attachment of ZnO NPs on bacteria and the morphological
changes such as disrupted cell wall integrity, cell bending and cell distortion as the result of
ZnO NPs interaction on the cell wall of P. aeruginosa.


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