Triple-enzymatic activity of CuMn2O4 nanoparticles: Analytical applications for H2O2 and L-cysteine detection

Document Type : Article


Department of Chemistry, Faculty of Sciences, University of Guilan, Rasht, P.O. Box 1914, Iran


CuMn2O4 NPs were prepared via coprecipitation method and characterized using microscopic and spectroscopic analyses. CuMn2O4 NPs exhibit a triple-enzymatic activity including peroxidase-, oxidase- and catalase-like activity. The effect of various parameters on the initial rate of the catalytic reaction of CuMn2O4 NPs with peroxidase- and oxidase-like activity was studied by UV-vis spectrometer following the increasing absorption at 415 nm corresponding to the oxidation product of substrate o-phenylenediamine (OPD). Kinetic analyses indicate the Michaelis-Menten model for CuMn2O4 NPs for both peroxidase- and oxidase-like activity. Based on the high peroxidase-like activity of CuMn2O4 NPs, they were further studied as a colorimetric sensor for the detection of H2O2 with a linear range from 0.5 mM to 22 mM and detection limit of 0.11 mM. Inhibition of the high oxidase-like activity of CuMn2O4 NPs was utilized for colorimetric detection of L-cysteine with a linear range from 50 µM to 200 µM and a detection limit of 54.15 µM.


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