Department of Chemical and Petrochemical Engineering,Sharif University of Technology
Biochemical and Bioenvironmental Research Center,Sharif University of Technology
The eect of glucose concentration in submerged fermentation (SMF) on chitosan
production by Aspergillus niger was investigated. A. niger, BBRC, 20004, from the Biochemical and
Bioenvironmental Research Centre at Sharif University of Technology, Tehran, Iran, was grown in a
Sabouro Dextrose media. Chitosan was extracted from the fungal mycelia using hot alkaline and acid
treatment and after 12 days of cultivation, 0.8455 g chitosan /l of the fermentation medium was obtained.
The content of glucose in the Sobouro Dextrose Broth media was also changed and the highest yield of
chitosan 0.9121g/l was obtained in Sobouro Dextrose Broth media containing 8% glucose.