Extraction solvent concentration affecting the anthocyanins and other phytochemicals profile and antioxidant properties of bran extracts of pigmented rice cultivars

Document Type : Article


Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, Punjab, 148106, India


Different concentrations (100%, 90% and 80%) of extracting solvent methanol were used to determine antioxidant capacities of seven pigmented rice cultivars bran on the basis of phytochemicals and other antioxidants. TPCs of red colored bran (Zag, Kaw quder, Shel kew) were highest in 100% methanol and that of black pigmented (Samarkand), lightly blackish (Kaw kareed) and brown (Gull zag, Teli zag) were highest in 80% and 90% methanol. The higher flavonoid contents in non-pigmented Gull zag and Teli zag were due to luteolin-7-O-glucoside, quercetinhexoside, apigenin-7-O-glucoside, quercetin-3-O-galactoside, apigenin and (epi)catechin rather than anthocyanins in pigmented rice. Higher anthocyanin contents of extracts in lower methanol concentration was due to higher percentage of Cyanidin-3-O-rutinoside, Pelargonidin-3-O-diglucoside, cyanidin-3-O-galactoside. The antioxidant activity had shown a similar trend in which the pigmented cultivars showed higher antioxidant activity in 100% methanol except red colored She lkew having higher value in 90% methanol, while among the light colored brown rice bran, Kaw kareed showed higher activities in 80% methanol and Teli zag in 90% methanol.


Main Subjects

1. Robert, Y., Shinji, T., and Naofumi, M. Identi cation
of phenolic compounds isolated from pigmented rice
and their aldose reductase inhibitory activities", Food
Chem., 101, pp. 1616-1625 (2007).
2. Kehrer, J.P. Free radicals as mediators of tissue injury
and disease", Critical Reviews in Toxicol., 23(1), pp.
21-48 (1993).
3. Park, Y.S., Kim, S.J., and Chang, H.I. Isolation
of anthocyanin from black rice Heugjinjubyeo and
screening of its antioxidant activities", J. Microbio.
Biotech., 36(1), pp. 55-60 (2008).
4. Biswas, S., Sircar, D., Mitra, A., and De, B. Phenolic
constituents and antioxidant properties of some
varieties of Indian rice", Nutrition Food Sci., 41, pp.
123-135 (2011).
5. Yodmanee, S., Karrila, T.T., and Pakdeechanuan,
P. Physical, chemical and antioxidant properties of
pigmented rice grown in Southern Thailand", Intern.
Food Research J., 18(3), pp. 901-906 (2011).
6. Min, S.W., Ryu, S.N., and Kim, D.H. Antiin

ammatory e ects of black rice, cyanidin-3-O- -Dglycoside,
and its metabolites, cyanidin and protocatechuic
acid", Intern Immuno Pharmocol., 10, pp. 959-
966 (2010).
7. Arab, F., Alemzadeh, I., and Maghsoudi. V. Determination
of antioxidant component and activity of rice
bran extract", Scientia Iranica, 18(6), pp. 1402-1406
8. Van der Sluis, A.A., Dekker, M., and Boekel, M.A.J.S.
Activity and concentration of polyphenolic antioxidants
in apple juice stability during storage", J.
Agricul. Food Chem., 53, pp. 1073-1080 (2005).
9. Cheng, Z., Su, L., Moore, J., Zhou, K., Luther,
M., Yin, J.J., and Yu, L.L. E ect of postharvest
treatment and heat stress on availability of wheat
antioxidants", J. Agricul. Food Chem., 54, pp. 5623-
5629 (2006).
10. Finocchiaro, F. Ferrari, B., and Gianinetti, A. A
study of biodiversity of
avanoid content in rice caryopsis
evidencing simultaneous accumulation of nanthocyanins
and proanthocyanins in a black-grained
genotype", J. Cereal Sci., 51, pp. 28-34 (2010).
11. Amerine, M.A. and Ough, C.S. Methods for analysis
of must and wines", Wiley-Interscience Publication,
New York, pp. 153-157 (1980).
12. Abu, B., Mohamed, M.F., Rahmat, A., and Fry, J.R.
Phytochemicals and antioxidant activity of di erent
parts of bambangan (Mangifera pajang) and tarap
(Artocarpus odoratissimus)", Food Chem., 113, pp.
479-483 (2009).
13. Hosseinian, F.S., Li, W., and Beta, T. Measurement
of anthocyanins and other phytochemicals in purple
wheat", Food Chem., 109(4), pp. 916-924 (2008).
14. Sanchez-Moreno, C., Larrauri J.A. and Saura-Calixto,
F. Free radical scavenging capacity and inhibition
of lipid oxidation of wines, grape juices and related
polyphenolic constituents", Food Res. Intern., 32, pp.
407-412 (1999).
15. Yen, G.C. and Duh, P.D. Antioxidative properties of
methanolic extracts from peanut hull", Journal of the
Am. Oil Chem. Soc., 70(4), pp. 383-386 (1993).
16. Khan, R.A., Khan, M.R., Sahreen, S., and Ahmed,
M. Assessment of
avonoids contents and in Vitro
antioxidant activity of Launaea Procumbens", Chem.
Cent. J., 6, pp. 1-11 (2012).
17. Badmus, J.A., Adedosu, T.O., Fatoki, J.O., Adegbite,
V.A., Adaramoye, O.A., and Odunola, O.A. Lipid
peroxidation inhibition and antiradical activities of
some leaf fractions of Mangifera Indica", Acta Pol.
Pharm., 68(1), pp. 23-29 (2011).
18. Fukumoto, L. and Mazza, G. Assessing antioxidant
and pro-oxidant activities of phenolic compounds", J.
Agricul. Food Chem., 48, pp. 3597-604 (2000).
19. Tian, X., Hansen, D., Schedl, T., and Skeath,
J.B. Epsin potentiates Notch pathway activity in
Drosophila and C. elegans", Development, 131(23),
pp. 5807-5815 (2004).
20. Gu, L., Kelm, M.A., Hammerstone, J.F., Beecher, G.,
Holden, J., Haytowitz, D., Gebhardt, S., and Prior,
R.L. Concentrations of proanthocyanidins in common
foods and estimations of normal consumption", J.
Nutr., 134(3), pp. 613-617 (2004).
21. Tan, P.W., Tan, C.P., and Ho, C.W. Antioxidant
properties: E ects of solid-to-solvent ratio on antioxidant
compounds and capacities of Pegaga (Centella
asiatica)", Intern. Food Res. J., 18, pp. 553-558
22. Butchan, K., Jirakanjanakit, N., and Jirasripongpun,
K. Antioxidant activities in bran extracts of black and
red rice", The 7th Silpakorn University International
Conference on Academic Research and Creative Arts:
Integration of Art and Science, pp. 285-290 (2013).
23. Tatiya, A.U., Ganesh, G., Tapadiya, S.K., and Sanjay,
J.S. E ect of solvents on total phenolics, antioxidant
and antimicrobial properties of (Bridelia Retusa
Spreng) stem bark", Indian J. Natu. Produ. Resor.,
2(4), pp. 442-447 (2011).
24. Shen, Y., Jin, L., Xiao, P., Lu, Y., and Bao, J.S. Total
avonoids, antioxidant capacity in rice grain
and their relations to grain color, size and weight", J.
Cereal Sci., 49(1), pp. 106-111 (2009).
25. Butchan, K., Nuananong, J., Malyn, C., and Kalyanee,
J. Anti-oxidative stress of red and black rice bran
extracts against H2O2, t-BHP, and UVA radiation",
The 26th Annual Meeting of the Thai Society for
Biotechnology and International Conference, pp. 319-
325 (2014).
F.M. Bhat and C.S. Riar/Scientia Iranica, Transactions C: Chemistry and ... 25 (2018) 3331{3344 3343
26. Tapas, A.R., Sakarkar, D.M., and Kakde, R.B.
Flavonoids as nutraceuticals", Trop. J. Pharmaceu.
Res., 7(3), pp. 1089-1099 (2008).
27. Naczk, M. and Shahidi, F. Extraction and analysis of
phenolics in food", J. Chromatogra. Analy., 1054, pp.
95-111 (2004).
28. Santa-Maria, C., Revilla, E., Miramontes, E.,
Bautista, J., Garcia-Martinez, A., Romero, E., Carballo,
M., and Parrado, J. Protection against free
radicals (UVB irradiation) of a water-soluble enzymatic
extract from rice bran. Study using human
keratinocyte monolayer and reconstructed human epidermis",
Food Chem. Toxicol., 48, pp. 83-88 (2010).
29. Shon, M.Y., Choi, S.D., Kohng, G.G., Nam, S.H., and
Sung, N.J. Antimutagenic, antioxidant and free radical
scavenging activity of ethylacetate extracts from
white, yellow and red onion", Food Chem. Toxicol.,
42, pp. 659-666 (2004).
30. Oki, T., Masuda, M., Kobayashi, M., Nishiba, Y.,
Furuta, S., Suda, I., and Sato. T. Polyemeric procyanidins
as radical-scavenging compounds in redhulled
rice", J. Agricul. Food Chem., 50, pp. 7524-7529
31. Iqbal, S., Bhanger, M.I., and Anwar, F. Antioxidant
properties and components of some commercially available
varieties of rice bran in Pakistan", Food Chem.,
93, pp. 265-272 (2005).
32. Gupta, S. and Prakash, J. Studies on Indian green
leafy vegetables for their antioxidant activity", Plant
Foods Human Nutrin., 64, pp. 39-45 (2009).
33. Medina, I., Gallardo, J.M., Gonzlez, M.J., Lois, S., and
Hedges, N. E ect of molecular structure of phenolic
families as hydroxycinnamic acids and catechins on
their antioxidant e ectiveness in minced sh muscle",
J. Agricul. Food Chem., 55, pp. 3889-3895 (2007).
34. Sasidharan, S., Chen, Y., Saravanan, D., Sundram,
K.M., and Latha, L.Y. Extraction, isolation and
characterization of bioactive compounds from plants
extracts", African J. Trad. Complemen. Alternative
Medicin., 8(1), pp. 1-10 (2011).
35. Zubair, M., Farooq, A., and Shaukat, A.S. E ect
of extraction solvents on phenolics and antioxidant
activity of selected varieties of pakistani rice (Oryza
sativa)", Intern. J. Agricul. Bio., 14(6), pp. 1814-9596
36. Nam, S.H., Choi, S.P., Kang, M.Y., Koh, H.J.,
Kozukue, N., and Griedman, M. Antioxidative activities
of bran extract from twenty one pigmented rice
cultivars", Food Chem., 94, pp. 613-620 (2006).
37. Pan, Y., Wang, K., Huang, S., Wang, H., Mu, X.,
He, C., Ji, X., Zhang, J., and Huang, F. Antioxidant
activity of microwave-assisted extract of longan (Dimocarpus
longan Lour) peel", Food Chem., 106, pp.
1264-1270 (2008).
38. Zhu, K.X., Lian, C.X., Guo, X.N., Peng, W., and
Zhou, H.M. Antioxidant activities and total phenolic
contents of various extracts from defatted wheat
germ", Food Chem., 126, pp. 1122-1126 (2011).
39. Natella, F., Belelli, F., Rambarti, A., and Scaccini, C.
Microwave and traditional cooking methods: E ect of
cooking on antioxidant capacity and phenolic content
of seven vegetables", J. Food Biochem., 34, pp. 796-
810 (2010).
40. Saenkod, C., Liu, Z., Huang, J., and Gong, Y. Antioxidative
biochemical properties of extracts from some
Chinese and Thai rice varieties", African J. Food Sci.,
pp. 1996-0794 (2013).
41. Gulcin, I., Buyukokuroglu, M.E., Oktay, M., and
Kufrevioglu, O.I.J. On the in vitro antioxidant properties
of melatonin" J. Pineal Res., 33, pp. 167-171
42. Porter, W.L. Paradoxical behavior of antioxidants in
food and biological systems", Toxicol. Indust. Health,
9, pp. 93-122 (1993).
43. Lizcano, L.J., Viloria-Bernal, M., Vicente, F.,
Berrueta, L.A., Gallo, B., Martinez-canamero, M.,
Ruiz-Larrea, M.B., and Ruiz-Sanz, J.I. Lipid oxidation
inhibitory e ects and phenolic composition of
aqueous extracts from medicinal plants of colombian
amazonia", Intern. J. Molec. Sci., 13, pp. 5454-5467