Sharif University of Technology Scientia Iranica 1026-3098 28 6 2021 12 01 Antibacterial properties of zinc oxide nanoparticles on Pseudomonas aeruginosa (ATCC 27853) 3806 3815 22560 10.24200/sci.2021.56815.4974 EN Kirbashini Dhanasegaran Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia Sinouvassane Djearamane Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia Sharolynne Xiao Tong Liang Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia Ling Shing Wong Department of Biotechnology, Faculty of Health and Life Sciences, INTI International University, Nilai, Negeri Sembilan, Malaysia Govindaraju Kasivelu Centre for Ocean Research, (DST-FIST sponsored Centre), Sathyabama Institute of Science and Technology, Chennai, India Poh Foong Lee Department of Mechatronics & Biomedical Engineering, Lee Kong Chian Faculty of Engeneering and Sciences, Universiti Tunku Abdul Rahman, Bandar Sungai Long, 43000, Malaysia Yang Mooi Lim Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Bandar Sungai Long, 43000, Malaysia Journal Article 2020 10 02 The involvement of nanotechnology has brought advancements in the environmental<br />and medical applications. Recently, zinc oxide nanoparticle (ZnO NP) is commonly used to<br />treat a wide range of bacterial and fungal skin infections due to its antimicrobial property.<br />This investigation was intended to study the antimicrobial effect of ZnO NP on Pseudomonas<br />aeruginosa by testing the bacterial inhibition and the morphological damages caused by ZnO<br />NP on P. aeruginosa. The results of the study at 24 h exhibited a typical dose dependant and<br />significant (p> 0.05) inhibition on the growth of P. aeruginosa treated with 5 to 150 μg/mL<br />of ZnO NP. The polysaccharides and polypeptides from P. aeruginosa cell wall were found to<br />be associated to the attachment of ZnO NPs on bacterial cells as illustrated in the Fourier<br />transform infrared (FTIR) spectrum. Furthermore, the scanning electron microscopy (SEM)<br />images displayed the surface attachment of ZnO NPs on bacteria and the morphological<br />changes such as disrupted cell wall integrity, cell bending and cell distortion as the result of<br />ZnO NPs interaction on the cell wall of P. aeruginosa. https://scientiairanica.sharif.edu/article_22560_e8d92556b83fd9af954274c6763ec64d.pdf