TY - JOUR ID - 4595 TI - A novel nano-composite scaffold for cartilage tissue engineering JO - Scientia Iranica JA - SCI LA - en SN - 1026-3098 AU - Mehdikhani-Nahrkhalaji, M. AU - Tavakoli, E. AU - Zargar-Kharazi, A. AU - Hashemi-Beni, B. AD - Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, 8174613441, Iran AD - Department of Biomaterials, Nanaotechnology and Tissue Engineering, School of Advanced technology in Medicine, Isfahan university of Medical sciences, Isfahan, 8174673461, Iran AD - Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 8174673461, Iran Y1 - 2018 PY - 2018 VL - 25 IS - 3 SP - 1815 EP - 1823 KW - Osteoarthritis (OA) KW - human Adipose-Derived Mesenchymal Stem Cells (hADMSCs) KW - PLGA KW - Tissue Engineering KW - Hybrid Scaffolds DO - 10.24200/sci.2017.4595 N2 - In this study, a hybrid Poly (lactic-co-glycolic acid) (PLGA)/ Hyaluronic acid (Ha)/ Fibrin/ 45S Bioactive Glass (45SBG) nanocomposite scaffolds seeded with human Adipose-Derived Mesenchymal Stem Cells (hADMSCs) were investigated as a construct for osteoarthritis (OA), articular cartilage (AC), and subchondral bone defects therapies. The bioactivity and biodegradation of the nanocomposite scaffolds were assessed in simulated body fluid (SBF) and phosphate buffer saline (PBS) solution, respectively. Furthermore, MTT analysis was performed in order to determine attachment and viability of hADMSCs. Ultimately, results indicated that bioactivity were increased in nanocomposite scaffolds as compared to the pure PLGA scaffold. As well as, biodegradation assay exhibited that the addition of Ha, fibrin, and 45SBG nanoparticles could modify the degradation rate of PLGA. The nanocomposite scaffolds were not showed any cytotoxicity and the hADMSCs were attached on the scaffolds and proliferate properly. According to our investigation, it was concluded that using natural and synthetic polymers along with BG nanoparticles may provide a suitable construct and could show a beneficial role in AC tissue engineering and OA therapy. UR - https://scientiairanica.sharif.edu/article_4595.html L1 - https://scientiairanica.sharif.edu/article_4595_bb0a7fcf7ee20a4ffc81d3442fa8257c.pdf ER -